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Synthesis of the exosporium during sporulation of Bacillus ..

Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of Bacillus cereus

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Synthesis of the exosporium during sporulation of Bacillus cereus ..

Both GerN and GerT are members of the CPA2 monovalent cation:proton antiporter family of membrane transport proteins () and are homologs of the NapA family of proteins. The GerN protein behaves as a Na+/H+-K+ antiporter and is essential for normal GerI receptor function, possibly in the restoration of local ion balance (). As yet, the transport capabilities of GerT have not been directly measured, but the NaCl and alkali sensitivity of the B. cereus gerT mutant during spore outgrowth and the ability of the cloned gerT gene to improve the growth of a Na+ transport-defective E. coli mutant in complementation studies both suggest a role in Na+ efflux. As described above, there is evidence for gerT expression during late sporulation, and GerT must be present in dormant spores, as it is responsible for the phenotypic difference between gerN spores and gerN gerT double mutant spores in germination at a stage before de novo protein synthesis. Whether preexisting GerT protein in the germinated spore is sufficient for outgrowth or whether in normal cells gerT is expressed again during outgrowth is not known.

Synthesis of the exosporium during sporulation of Bacillus cereus…

Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of Bacillus cereus. An antiserum to a soluble extract of exosporium from mature spores was used to analyse extracts of cells from various stages of the Bacillus life cycle by the technique of one-dimensional immunoelectrophoresis. Exosporium antigens were absent from vegetative cell extracts and were first detected in extracts of cells at stage III of sporulation, where they appeared simultaneously in the soluble and particulate cellular fractions. The results indicate that the exosporium contains antigens which are spore specific.

21/12/2017 · Synthesis of the Exo..

Sporulation temperature reveals a requirement for CotE in the assembly of both the coat and exosporium layers of Bacillus cereus spores. 04 November 2015

The exosporium is the outermost layer of spores of Bacillus cereus and its close relatives Bacillus anthracis and Bacillus thuringiensis. For these pathogens, it represents the surface layer that makes initial contact with the host. To date, only the BclA glycoprotein has been described as a component of the exosporium; this paper defines 10 more tightly associated proteins from the exosporium of B. cereus ATCC 10876, identified by N-terminal sequencing of proteins from purified, washed exosporium. Likely coding sequences were identified from the incomplete genome sequence of B. anthracis or B. cereus ATCC 14579, and the precise corresponding sequence from B. cereus ATCC 10876 was defined by PCR and sequencing. Eight genes encode likely structural components (exsB, exsC, exsD, exsE, exsF, exsG, exsJ, and cotE). Several proteins of the exosporium are related to morphogenetic and outer spore coat proteins of B. subtilis, but most do not have homologues in B. subtilis. ExsE is processed from a larger precursor, and the CotE homologue appears to have been C-terminally truncated. ExsJ contains a domain of GXX collagen-like repeats, like the BclA exosporium protein of B. anthracis. Although most of the exosporium genes are scattered on the genome, bclA and exsF are clustered in a region flanking the rhamnose biosynthesis operon; rhamnose is part of the sugar moiety of spore glycoproteins. Two enzymes, alanine racemase and nucleoside hydrolase, are tightly adsorbed to the exosporium layer; they could metabolize small molecule germinants and may reduce the sensitivity of spores to these, limiting premature germination.

AB - Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of Bacillus cereus. An antiserum to a soluble extract of exosporium from mature spores was used to analyse extracts of cells from various stages of the Bacillus life cycle by the technique of one-dimensional immunoelectrophoresis. Exosporium antigens were absent from vegetative cell extracts and were first detected in extracts of cells at stage III of sporulation, where they appeared simultaneously in the soluble and particulate cellular fractions. The results indicate that the exosporium contains antigens which are spore specific.

25/12/2001 · Characterization of the exosporium of ..

Summary: Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of Bacillus cereus

N2 - Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of Bacillus cereus. An antiserum to a soluble extract of exosporium from mature spores was used to analyse extracts of cells from various stages of the Bacillus life cycle by the technique of one-dimensional immunoelectrophoresis. Exosporium antigens were absent from vegetative cell extracts and were first detected in extracts of cells at stage III of sporulation, where they appeared simultaneously in the soluble and particulate cellular fractions. The results indicate that the exosporium contains antigens which are spore specific.

SYNTHESIS OF THE EXOSPORIUM DURING SPORULATION OF BACILLUS CEREUS. We are proud of ensuring individual approach to every customer who …
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  • Growth and Sporulation of Bacillus cereus ATCC 14579 …

    Abstract

  • Characterization of the exosporium of Bacillus cereus ..

    03/02/2017 · Growth and Sporulation of Bacillus cereus ATCC 14579 ..

  • Bacillus cereus family revealed by electron crystallography

    Molecular Microbiology

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