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Genetics : PrimeScript™ II 1st Strand cDNA Synthesis Kit

And while performing the QC assay, proceed to second strand cDNA synthesis with up to 16 samples to ensure sample stability.

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Second Strand cDNA Synthesis | NEB

With the QuantSeq-Flex Second Strand Synthesis Module the random second strand synthesis primer can be substituted with a target specific primer in the QuantSeq protocol.

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based (25 x 50 µl reactions)

With the QuantSeq-Flex Second Strand Synthesis Module the random second strand synthesis primer can be substituted with a target specific primer in the QuantSeq protocol.

First Strand cDNA Synthesis Kit - Fisher Scientific

First Strand cDNA Synthesis Kit First Strand cDNA Synthesis Kit Direct PCR Reagents and Kits

In general 1 ng to 1 μg total RNA or 0.1-100 ng mRNA arerecommended.

First Strand cDNA SynthesisReaction
Denaturation of RNA and primer at 70°C for 5 minutes canremove secondary structures that may impede long cDNA synthesis.

The first strand cDNA productgenerated is more than 10 kb (Figure 1).

General Information forSuccessful cDNA Synthesis:

Template RNA
Intact RNA of high purity is essential for sensitive RT-PCR detection.

Synthesis of second-strand cDNA by ..

a component in some cDNA synthesis kits, ..

Thermo Scientific™ RevertAid™ First Strand cDNA Synthesis …
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    The PrimeScript II 1st Strand cDNA Synthesis Kit contains all of the reagents necessary to synthesize ..

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    A complete system for efficient synthesis of first strand cDNA from ..

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