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PaperChromatography | Chlorophyll | Chromatography

In this lab a red, green and brown algae will be used to extract the photosynthetic pigments from.

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Photosynthesis Essays and Research Papers | …

ABSTRACT: The relationships between soil nutrients uptake, leaf glucose contents, and chlorophyll contents of Pelargonium sidoides was investigated. Pelargonium sidoides is a medicinal plant grown in Kenya for export. It is used in the manufacture of drugs for lung related diseases. P. sidoides plants were selected at a field site, where the soil N, P, K and Ca contents had prior been analysed. These plants were dressed with both inorganic (Calcium Ammonium Nitrate, Triple Super Phosphate, Calcium Carbonate, Potassium Nitrate) and organic fertilizers (worm leachate) of known nutrient levels. Leaf glucose contents before and after application of the mineral nutrients were measured by colorimetry. Verification of the colorimetric results was done by high performance liquid chromatography fitted with a refractive index detector (HPLC). Predictions for nutrients in soil and plant tissues were done by DRIFT FTIR. Leaf chlorophyll measurements were done by Soil Plant Analysis Development units (SPAD). The mean glucose contents before and after each nutrients application were subjected to GENSTAT statistical software for the ANOVA and t-test to find out whether there were any significant differences between the two. The results indicated that the levels of leaf glucose increased with type and uptake of certain applied fertilizers. FTIR predictions closely matched the wet chemistry data. Uptake of calcium corresponded to the highest increase of glucose while that of worm leachate had the least effect. Change in glucose levels was evident within an hour after application, suggesting the possibility of predicting nutrients uptake within a short period. Chlorophyll content was also found to increase with nutrients uptake. All plants treated with fertilizers displayed significantly higher glucose contents in comparison to the control samples. The study opened way for real time analysis of plant status and allowed for real time interventions where necessary.
Key words:
nutrients uptake, leaf glucose content, chlorophyll content, organic and inorganic fertilizers

 Then we can find out what wavelength of light a plant uses most to carry out photosynthesises.

The diagram below shows a setup that may be useful. It really just show the connection of two things: a flask with a sidearm (maybe a Büchner flask) and a graduated glass syringe. The exact positioning is something you should determine. Glass syringes are precision-made with low friction between the plunger and the barrel (unlike plastic ones that have high friction). Your should have some in the chem lab and if not they are reasonably cheap (about $50 for a 100 mL one). You need to introduce a gas (eg CO2) into the flask and surround the flask with water in a beaker on a hotplate. As it slowly heats (I mean slowly, maybe 20°C to 80°C over 40 minutes) the gas expands and the syringe is pushed out. With the syringe on it's side there is no need to worry about the weight of the plunger. You could compare gases - oxygen, nitrogen, hydrogen for example.

Plant Pigments and Photosynthesis

When the solvent “front” is within 2-3cm from the top of the paper, remove the chromatogram.

We will also be extracting the photosynthetic pigments from Coleus sp., which is a red land plant leaf and, and compare the accessory pigments of this to the red algae....

(A more thorough discussion of the light-absorbing properties of
pigments will be presented in the Spectrophotometry lab.) The broad array of colors found in
plant tissues such as leaves, flowers, and fruits, can be accounted for by the presence of
literally thousands of different kinds of plant pigments.
Through plant breeding and horticultural practices, humans have manipulated plants’ pigment
producing capabilities to serve our own desires.

also found in green plants by the process of Photosynthesis

Many lines of research
suggest that consuming a diet rich in plant pigments may slow the process of cellular aging and
reduce the risks of some types of disease, such as cancer, heart disease, and stroke.

Handle the papers by the
edges, taking care to touch them as little as possible – oils from fingertips can interfere with
the migration of pigments up the paper.
Step 2: Prepare your plant extract.

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  • absorb light that is used in photosynthesis.

    Recognition for research work, The Tasmanian Country Newspaper, Hobart, Tasmania, November (2001) [Newspaper Article]

  • EXPERIMENT 9 PHOTOSYNTHESIS: Pigments of the Chloroplasts

    Fred EB, Wilson PW. 1934. On photosynthesis and free nitrogen assimilation by leguminous plants. 20: 403-09

  • Photosynthesis uses energy absorbed by several ..

    Paper Chromatography of ..

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Chlorophyll Extraction - Science Buddies

I would recommend not
taking out the pigment until the last minute when it is needed in
order to minimise the effect of light;

l An alternative technique (thin layer chromatography) mentioned in
the preparation paper can be employed: instead of absorptive paper, a
glass of plastic slide (or aluminium sheet) coated with silica gel is

Paper chromatography science project to extract ..

The gel is spotted and then the slide or aluminium sheet is
placed vertically in a beaker or Coplin jar containing solvent at the

l Further experiment can employ pigments from different plants.

Diffusion | Cell Membrane | Photosynthesis

Make sure that the level of the solvent is
below the origin on your chromatogram – you do not want to submerge the origin in the solvent.
Check the chromatogram frequently to observe the movement of solvent and pigment up the
chromatography paper.
WARNING: The petroleum ether/acetone solvent is highly flammable and can be dangerous if


During peak fruit expansion, usually early summer, there is an intense flow of photoassimilate from mature leaves (sources) into rapidly enlarging fruit (sinks). Sugars generated by photosynthesis, along with amino acids and phosphate within the plant’s vascular network, move via the phloem into enlarging fruit.


Due to the great damage can be done by the scale insects not only by sucking the plant sap that give low photosynthesis and respiration, which leads to curling, yellowing, dropping to leaves, so malformation, dwarfing and decreasing fruit production ( quality and quantity ),even plant death; but also due to the excretion of large amount of honey dew th...

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