Synthetic Metals - Journal - Elsevier
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Cavaillé J, Chetouani F and Bachellerie JP (1999) The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2′‐O‐ribose methyltransferase catalyzing the formation of Gm18 in tRNAs. RNA 5: 66–81.
Automated synthesis reduces the radiation exposure to the operator, improves robustness of the production as well as provides on-line documentation of the manufacturing process thus improving GMP compliance. Considerable number of semi-automated and fully automated devices with either stationary tubing system or disposable cassettes for the automated production of radioactive probes is available on the market as well as built “in house” [-]. They offer application of three most common methods: fractionation and preconcentration/prepurification of the generator eluate using either cation or anion exchange resins. Synthesizers based on the disposable sterile cassettes and devoted to the production of 68Ga-comprising imaging agents are most preferred in routine clinical setup even though the cost in higher. They exclude the risk of cross-contamination, improve the reproducibility and reduce the radiation burden upon the operator. Both hardware and software should comply with GMP and good automated manufacturing practice 5 (GAMP5). The automation may provide possibility for the harmonized and standardized multicentre clinical studies that in turn would accelerate the introduction of new radiopharmaceuticals as well as their regulatory approval. This in turn will motivate investments into the research and development of novel 68Ga-based radiopharmaceuticals.
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Doxycycline is a wide spectrum antibacterial synthetically derived from oxytetracycline that is generally superior in our aquatic environments.
Doxycycline also has been shown to have delayed anti-malarial activity, which is a similar pathogen to Oodinium & Ich found in our aquarium pond fish. However this is not a "best product" for this use in our aquarium fish
The most common radionuclides relevant for the labelling synthesis of radiopharmaceuticals for PET, SPECT, and radiotherapy are presented in Table . The majority are metals typically involved in coordination labelling chemistry. Only few of the radionuclides are produced in generator systems. Such factors as production mode as well as physical and chemical characteristics determine the choice of a radionuclide.
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Roovers M, Oudjama Y, Fislage M et al. (2012) The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N²‐methylguanosine at position 6 in tRNA. RNA 18: 815–824.
Noma A, Kirino Y, Ikeuchi Y and Suzuki T (2006) Biosynthesis of wybutosine, a hyper‐modified nucleoside in eukaryotic phenylalanine tRNA. EMBO Journal 25: 2142–2154.
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Definition of synthesis: Systematic combination of otherwise different elements to form a coherent whole.
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A multi-step synthesis of any organic compound requires the chemist to accomplish three related tasks: 1
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Globin synthesis refers to the expression of theα‐like and β‐like globin genes in red blood cells
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Miller DL and Martin NC (1983) Characterization of the yeast mitochondrial locus necessary for tRNA biosynthesis: DNA sequence analysis and identification of a new transcript. Cell 34: 911–917.
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Shigi N, Sakaguchi Y, Asai S, Suzuki T and Watanabe K (2008) Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur‐containing cofactors. EMBO Journal 27: 3267–3278.
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Requiring more transparent research practices, with reasonable exceptions for difficult situations, is possible. require open data, research materials, and analytic code, while encouraging disclosure of additional steps that can increase the credibility of inferential research.
Prospective of 68Ga-Radiopharmaceutical Development
Warner GJ, Berry MJ, Moustafa ME et al. (2000) Inhibition of selenoprotein synthesis by selenocysteine tRNA[Ser]Sec lacking isopentenyladenosine. Journal of Biological Chemistry 275: 28110–28119.
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Ikeuchi Y, Shigi N, Kato J, Nishimura A and Suzuki T (2006) Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Molecular Cell 21: 97–108.
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One of the most rapidly expanding areas is the development of peptide-based agents for targeted imaging. Metal and halogen radionuclides such as 64Cu, 89Zr, 99mTc, 111In, 18F, and 124I are most commonly used for the design of the agents. Typically, the metal ion coordination chemistry is straightforward and mild. However, it requires attachment of a rather bulky chelator to the vector molecule what may deteriorate the biological activity. Simple direct iodination has been used for decades introducing minor modification at tyrosine amino acid residue though demonstrating poor residualizing of the radioactive iodine catabolites in the cell. On the contrary hydrophilic complexes of radiometals stay trapped in the cell after the degradation of the internalized imaging agent. The incorporation of 18F can be accomplished by the conventional nucleophilic or electrophilic addition, or via prosthetic groups . However, the synthesis might be rather complex with harsh conditions and high macromolecule concentration. A novel method utilizing similarity of Al18F2+ with metal cations and thus possibility of coordination labelling chemistry has potential for kit type production like radiometals . The relatively long half-life (110 min) of 18F enables centralized production and distribution of either the radionuclide or prepared imaging agents to the satellite clinical centres. Most of the PET agents used in clinical studies are 18F-based. Nevertheless, the simpler labelling chemistry of 68Ga and its availability from the generator system which can be used even at distant and isolated medical centres make 68Ga more preferable.
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