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Determine the effect of dnp on the atp synthesis

effect of dinitrophenol on a the production of ATP ..

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2,4-Dinitrophenol (2,4-DNP or simply ..

In order to study the relationship between bladder contraction and intracellular ATP, we characterized the effect of 2,4 dinitrophenol (DNP) on rabbit urinary bladder adenine nucleotide metabolsim, intracellular ATP concentration, and isolated bladder strip contraction.

Synthesis DNP is produced by ..

In rabbit ventricular myocytes, either pinacidil or the mitochondrial uncoupler dinitrophenol (DNP), which rapidly switches mitochondria from net ATP synthesis to net ATP hydrolysis, had little immediate effect on IK,ATP.

The effect of 2,4-dinitrophenol on adipose-tissue metabolism.

The effect of 2,4-dinitrophenol (DNP) on the synthesis of p-aminohippurate (PAH) by rat liver slices under several experimental conditions was studied.

In rabbit ventricular myocytes, either pinacidil or the mitochondrial uncoupler dinitrophenol (DNP), which rapidly switches mitochondria from net ATP synthesis to net ATP hydrolysis, had little immediate effect on IK,ATP.

Dinitrophenol (1 x 10-5 ) has been found to inhibit anaerobic sodium transport by the isolated urinary bladder of the fresh water turtle. Concurrently, anaerobic glycolysis was stimulated markedly. However, tissue ATP levels diminished only modestly, remaining at approximately 75% of values observed under anaerobic conditions without DNP. The utilization of glucose (from endogenous glycogen) corresponded closely to that predicted from the molar quantities of lactate formed. Thus the glycolytic pathway was completed in the presence of DNP and if ATP were synthesized normally during glycolysis, synthesis should have been increased. On the other hand, the decrease in Na transport should have decreased ATP utilization. Oligomycin did not block sodium transport either aerobically or anaerobically, but ATP concentrations did decrease. When anaerobic glycolysis was blocked by iodoacetate, pyruvate did not sustain sodium transport thus suggesting that no electron acceptors were available in the system. Two explanations are entertained for the anaerobic effect of DNP: (a) Stimulation by DNP of plasma membrane as well as mitochondrial ATPase activity; (b) inhibition of a high energy intermediate derived from glycolytic ATP or from glycolysis per se. The arguments relevant to each possibility are presented in the text. Although definitive resolution is not possible, we believe that the data favor the hypothesis that there was a high energy intermediate in the anaerobic system and that this intermediate, rather than ATP, served as the immediate source of energy for the sodium pump.

13/02/2017 · The effect of dinitrophenol on the ..

04/09/2009 · Dinitrophenol (DNP) is an uncoupler, or has the ability to separate the flow of electrons and the pumping of H+ ions for ATP synthesis

Dinitrophenol (DNP) is an uncoupler, or has the ability to separate the flow of electrons and the pumping of H + ions for ATP synthesis.
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  • The effect of 2,4-dinitrophenol on adipose-tissue metabolism

    2,4-Dinitrophenol - Wikipedia

  • The effect of dinitrophenol on ..

    09/02/2013 · How would exposure to DNP affect ATP synthesis by mitochondria

  • ( d ) in the presence of dinitrophenol, there is an excess of ATP ..

    Extra challenge: DNP raises body temperature and leads to rapid weight loss

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