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Biosynthesis of silver nanoparticles from ..

13-7-2017 · Biosynthesis of silver nanoparticles from Spirulina microalgae and its antibacterial activity

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Biosynthesis of metallic silver nanoparticles has ..

ABSTRACT:The synthesis of nanoparticles has become the matter of great interest in recent times due to its various advantageous properties and applications in various fields. Though physical and chemical methods are more popular for nanoparticle synthesis, the biogenic production is a better option due to eco-friendliness. Morinda coreia leaf ethanol extracts was screened for the synthesis of Silver nanoparticle using 0.1mM concentration of silver nitrate. The peak of 482 nm in UV-Vis Spectroscopy confirmed the presence of silver nanoparticle synthesis. Biosynthesized silver nanoparticles characterized by Scanning Electron Microscope (SEM), Fourier Transform Infra Red Spectroscopy (FT-IR) and Energy-dispersive X-ray spectroscopy (EDX). The silver nanoparticles around 90 nm were formed. Biologically synthesized silver nanoparticles were further examined for antimicrobial activity against both Gram-negative and Gram-positive bacteria. It was observed that a clear zone of growth inhibition was against in E. coli, Vibrio cholerae, Staphylococcus aureus, Bacillus sps, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Proteus vulgaris, Staphylococcus mutans and Salmonella typhi confirms the antibacterial property of biologically synthesized nanoparticles

04/08/2017 · Green Synthesis of Silver Nanoparticles and its Antibacterial ..

The development of appropriate processes for the synthesis of nanoparticles is an important aspect of nanotechnology. In this study, fungus mediated synthesis of silver nanoparticles using Microporus Xanthopus has been done. The dried macro fungi were powdered and boiled for 20 minutes to get the extracts. The extracts were treated with silver nitrate and kept in the dark conditions for 48 hrs, for the appearance of colour change. The Plasmon peak was observed at 425 nm. These nanoparticles were examined for the stability for a period of 2 months and were further characterized by SEM and FTIR. SEM analysis revealed the spherical nature of silver nanoparticles and size distributed in the range of 40 nm. However further studies regarding XRD, SEM, EDX, are confirmed to strengthen the existing findings. The synthesized silver nanoparticles have been investigated against the pathogenic bacteria Bacillus subtilis , Staphylococcus aureus, Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae and shigella sp for their antibacterial activity and minimum inhibitory concentration

of the silver nanoparticle compared to the Gram ..

‘Silver nanoparticle production by Rhizopus stolonifer and its antibacterial activity against ..

Biosynthesis of Ag nanoparticles (AgNPs) by extract was investigated. The AgNPs were characterized by ultraviolet–visible absorption spectroscopy (UV–vis), transmission electron microscopy (TEM), selected-area electron diffraction (SAED), and X-ray diffraction (XRD). The results showed that increasing the initial AgNO3 concentration at 30 or 60 °C increased the mean size and widened the size distribution of the AgNPs leading to red shift and broadening of the Surface Plasmon Resonance absorption. The conversion of silver ions was determined by atomic absorption spectroscopy (AAS) and to discuss the bioreductive mechanism, the reducing sugar, flavonoid, saccharide, protein contents in the extract, and the antioxidant activity were measured using 3,5-dinitrosalicylic acid colorimetric; Coomassie brilliant blue; phenol-sulfuric acid; rutin-based spectrophotometry method; and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging assay methods. The results showed that the reducing sugars and flavonoids were mainly responsible for the bioreduction of the silver ions and their reductive capability promoted at 90 °C, leading to the formation of AgNPs (18.4 ± 4.6 nm) with narrow size distribution. Finally, the antibacterial activity of the AgNPs against and was assessed to determine their potential applications in silver-loaded antibacterial materials. This work provides useful technical parameters for industrialization of the biosynthetic technique and further antibacterial application of the AgNPs. Furthermore, the elucidation of bioreductive mechanism of silver ions by measuring the change of the biomolecular concentrations in plant extract exemplifies understanding the formation mechanism of such biogenic AgNPs.

Its bark is corky. Its leaves are pale brown, long fissured elliptic or lanceolate. Its flowers are indense ovoid heads and white scented. Its fruit is drupe, globes or ovoid, ca.2 cm, in diameter and is edible. The leaves are usually 4.8 cm broadly or narrowly elliptic acute on both ends; glabrous or pubescent; peduncles solitary or 2-nate leaf-opposed a rarely terminal; flowers 5-merous; fruit of many drupes7. In the present study, we have demonstrated a suitable green method for the synthesis of silver nanoparticles using ethanolic leaf extract of Morinda coreia as reducing agent. The antibacterial activity of silver nanoparticles has been tested against various pathogenic bacteria.

peel extract and its antibacterial activity.

The AgNPs exhibited good antibacterial activity against both Gram-negative and Gram-positive bacteria. In the in-vitro antimicrobial assay, chloramphenicol, an antimicrobial agent that is widely used against many bacterial infections, was used as positive control. The antibacterial effects of biologically synthesized silver nanoparticles have been investigated against E. coli, Vibrio cholerae

This paper reports a rapid and ecofriendly green method for the synthesis of silver nonoparticles from silver nitrate solution using leaves extract. Effect of leaves extract, silver nitrate concentration, reaction time and temperature on reaction rate were investigated. The synthesized silver nanoparticles (AgNPS) were characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and fourier infrared spectroscopy (FT-IR). UV-vis spectra gave surface plasmon resonance (SPR) at 430 nm. This reveals the reduction of silver ions (Ag+) to silver (Ago) which indicates the formation of silver nanoparticles (AgNPs). The antibacterial activity of green synthesized AgNPs showed effective inhibitory activity against water borne pathogens This reveals that silver nanoparticles could provide a safer alternative to conventional antimicrobial and antibacterial agents.

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  • leaf extracts and its antibacterial activity against ..

    Biosynthesis of silver nanoparticles from streptomyces spp and its antimicrobial, anticancerous activity

  • biosynthesis of silver nanoparticle s.

    06/03/2016 · Biosynthesis of silver nanoparticle and its antibacterial ..


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Comparable aspects occurred with the antibacterial activity of silver nanoparticles. One such feature proven for silver nanoparticles is that for antibacterial activity, size, morphology and concentrations are all important. For example, small particles have larger surface areas to be in contact with the bacterial cells, showing a larger activity10. The antimicrobial efficacy of the nanoparticles also depends on the shape or morphology of the nanoparticles. Ingle has evaluated the antibacterial activity of biosynthesized silver nanoparticles produced by Fusarium acuminatum on different human pathogens. These authors reported efficient antibacterial activity of AgNps against multidrug resistant and highly pathogenic bacteria, such as, S. aureus, Salmonella typhi and E. coli. Silver nanoparticles showed significant antimicrobial effects than silver ions (1.4–1.9 folds). The maximum antibacterial activity of silver nanoparticles was against S. aureus, followed by Vibbrio cholera, S. typhi and the minimum was for E. coli Fig. 7. This result revealed that specific efficiency of silver nanoparticles can be related with differences due to the strain, which can be related to the bacterial membrane structure11.

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