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Pupa - Wikipedia
CD24 Gene - GeneCards | CD24 Protein | CD24 Antibody
The procedure presented here was developed to permit synthesis of cDNA using the TS-PCR approach from very small quantities of starting material, such as manually selected glandular trichomes or any sample of a particularly limited nature. cDNAs are synthesized using an adaptation of the SuperSMART approach relative to the sample handling. However, the primers used are similar to those from the SMART cDNA synthesis kit to enable SfiI digestion of the cDNA and ligation into the pDNR-LIB vector for library propagation in E. coli and subsequent Sanger sequencing. Digestion with SfiI also allows for cDNA produced in this way have most of the primer sequence removed prior to use in massively parallel sequencing approaches (next-generation sequencing) such as 454 Life Sciences GS FLX Titanium series sequencing. Validation of the utility of this method in such approaches was provided by successful construction and 454 sequencing of cDNA from rhizome samples of two divergent plant species: scouring rush (fern ally, "primitive plant") and red rice (monocot, "advanced plant"). We have continued to use this method to produce cDNA samples from several other plant species with similarly successful results in next-gen sequencing.
Mint-2 cDNA synthesis kit is designed to synthesize full-length-enriched double stranded (ds) cDNA from total or poly(A)+ RNA. Synthesized cDNA can be used for construction of cDNA libraries, subtractive hybridization (SSH), high-throughput sequencing on the next generation sequencing platforms (Roche/454, ABI/SOLiD or Illumina/Solexa), and other applications.
YY1 Gene - GeneCards | TYY1 Protein | TYY1 Antibody
Our results demonstrate that this novel approach for cDNA synthesis has valuable utility for application of ultra-high throughput technologies, such as whole transcriptome sequencing using 454 technology, to very small biological samples comprised of tens of cells as might be obtained via approaches like laser microdissection.
Evidence of the efficacy of the iso3TS oligo can be observed in comparisons of several of the EST libraries we have recently made and sequenced using 454 technology. For instance, in the Salvia divinorium, Solanum lycopsericum, and Solanum habrochaites glandular trichome libraries, we found that 20.6%, 17.9%, and 11.6% of the reads, respectively, contained 2 or more concatamers of the SMART IV-derived oligo (TS oligo). Many of these reads contained multiple concatamers as illustrated in Figure . This number of concatamers is dramatically reduced or eliminated via the use of the iso3TS oligo as seen in the Equisetum hyemale, Solanum habrochaites and Isatis tinctoria 454 libraries which possess 0.02%, 1.5%, 3.1% of reads possessing 2 or more concatamers of the SMART IV-derived oligo (TS oligo) (Table ), with the vast majority of these possessing only 2 concatamers. In all of these cases, the same method for cDNA synthesis, 454 library construction and sequencing was used, and involved the same amount of template RNA. With the modified method, we also produced high quality cDNA from rhizome tips and elongation zones of scouring rush and red rice that was suitable for next generation sequencing using the 454 GS-FLX instrument with Titanium technology (Figure ).
15/12/2004 · PLANT GENETICS REVIEW ARTICLE
RECENT PUBLICATIONS - Toyama Prefectural University
cDNA Synthesis, Library, & Cloning Kits
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Evrogen Mint-2 cDNA synthesis kit: Method overview
Oriental Journal of Chemistry is a peer reviewed quarterly research journal of pure and applied chemistry
Evrogen cDNA synthesis service: Summary
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